![]() Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. CLC Genomics Workbench is a cross-platform desktop application and is compatible with Windows, Mac OS X, and Linux platforms.The Long Read Support (beta) plugin is a collection of tools developed for working with long, error-prone reads such as those produced by the single-molecule sequencing technologies of Pacific Biosciences or Oxford Nanopore Technologies. Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. This tutorial is an introduction to working with the tools in the Long Read Support (beta) plugin.Koch CM, Chiu SF, Akbarpour M, Bharat A, Ridge KM, Bartom ET, Winter DR (2018) A Beginner’s guide to analysis of RNA sequencing data. Topics Brief introduction to RNA-Seq experiments Analyze RNA-seq data Download seq reads from EBI-ENA/NCBI SRA Import reads to CLC Genomics Workbench Align.For prokaryotes, annotations of type gene are considered. The key annotation types for RNA-Seq analysis of eukaryotes are of type gene and type mRNA. Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-seq. RNA-Seq analysis Based on an annotated reference genome and mRNA sequencing reads, the CLC Genomics Workbench is able to calculate gene expression levels as well as discover novel exons.Byron SA, Van Keuren-Jensen KR, Engelthaler DM, Carpten JD, Craig DW (2016) Translating RNA sequencing into clinical diagnostics: opportunities and challenges.Eric Seiser (Field Application Scientist QIAGEN) If you want to suggest a change, please let us know. Ozsolak F, Milos PM (2011) RNA sequencing: advances, challenges and opportunities. Date and Time: (10:00 am - 3:30 pm) Location: NIH Library Training Room.Royce TE, Rozowsky JS, Gerstein MB (2007) Toward a universal microarray: prediction of gene expression through nearest-neighbor probe sequence identification.Okoniewski MJ, Miller CJ (2006) Hybridization interactions between probesets in short oligo microarrays lead to spurious correlations.van Hal NL, Vorst O, van Houwelingen AM, Kok EJ, Peijnenburg A, Aharoni A, van Tunen AJ, Keijer J (2000) The application of DNA microarrays in gene expression analysis.Wang Z, Gerstein M, Snyder M (2009) RNA-seq: a revolutionary tool for transcriptomics.
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